Review



rabbit gnl3  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology rabbit gnl3
    Rabbit Gnl3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+gnl3/Nucleostemin+Antibody/pm37965896-539-23-26
    Average 93 stars, based on 29 article reviews
    rabbit gnl3 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    esiRNA:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Transfection:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Immunofluorescence:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    High Throughput Screening Assay:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Microscopy:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Negative Control:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Western Blot:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Labeling:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    MANN-WHITNEY:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Expressing:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Fractionation:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Inhibition:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Phospho-proteomics:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Standard Deviation:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Control:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Mass Spectrometry:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Immunoprecipitation:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    ChIP-sequencing:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Proximity Ligation Assay:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Quantitative Proteomics:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Two Tailed Test:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Comparison:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Chromatin Immunoprecipitation:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Sequencing:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Binding Assay:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Staining:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Extraction:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Activation Assay:

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: 1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).1 mM PMSF, 1 mM MgCl2 and Benzonase Nuclease 250 units/10 millions of cells (E1014-25KU, Sigma).. Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120-201, Bethyl Laboratories), rabbit GNL3 (sc-166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302-734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: Whole‐cell extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma).extracts of K562 cells were prepared using lysis buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 5 mM EDTA pH8, 0.5% NP40) supplemented with protease inhibitor cocktail (Roche), 1 mM PMSF, 1 mM MgCl 2 and Benzonase Nuclease 250 units/10 millions of cells (E1014‐25KU, Sigma). ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG) (P120‐201, Bethyl Laboratories), rabbit GNL3 (sc‐166460, Santa Cruz Biotechnology) or rabbit ORC2 antibody (A302‐734A, Bethyl Laboratories).. Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.Beads were washed four times with lysis buffer, then washed three times with 50 mM Tris HCl pH8.



    Similar Products

    rabbit gnl3  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology rabbit gnl3
    Rabbit Gnl3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+gnl3/Nucleostemin+Antibody/pm37965896-539-23-26
    Average 93 stars, based on 1 article reviews
    rabbit gnl3 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    rabbit anti-human gnl3 polyclonal antibody  (GeneTex)
    90
    GeneTex rabbit anti-human gnl3 polyclonal antibody
    Identification of TSC-22-binding proteins in cells before and after DNA damage. ( A ): TSC-22-binding protein (arrow) in the nuclear fraction after DNA damage by phleomycin in cells expressing full-length TSC-22. a: Lamin-B1, Heat shock 70-kDa protein 1A/1B, b: Guanine nucleotide-binding protein-like 3 <t>(GNL3),</t> c: Pituitary adenylate cyclase-activating polypeptide type I receptor, d: Charged multivesicular body protein 4a, Histone H1.2, Heterogeneous nuclear ribonucleoprotein A1, rRNA 2′-O-methyltransferase fibrillarin, *Flag-TSC-22. ( B ): TSC22(86)-binding proteins (←) after DNA damage by phleomycin in a whole cell fraction of cells expressing TSC22(86). a: Keratin, type II cytoskeletal 1, Ubiquitin carboxyl-terminal hydrolase 47, Protein tyrosine phosphatase receptor type C-associated protein, b: Keratin, type I cytoskeletal 9, Keratin, type II cytoskeletal 1, c: Histone H1.4, d: Histone H1t, Histone H1.2, e: Actin, cytoplasmic 1, f: Actin, cytoplasmic 1, Histone H1.2, Glyceraldehyde-3-phosphate dehydrogenase, g: Histone H1.2, Dermcidin, h: 40S ribosomal protein S6, Histone H1.2, Dermcidin, 40S ribosomal protein S2, *Flag-TSC22(86).
    Rabbit Anti Human Gnl3 Polyclonal Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+gnl3/rabbit+anti+human+gnl3+polyclonal+antibody/pmc08536140-170-58-63
    Average 90 stars, based on 1 article reviews
    rabbit anti-human gnl3 polyclonal antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    anti nucleostemin ns rabbit pabs  (Bethyl)
    93
    Bethyl anti nucleostemin ns rabbit pabs
    Identification of TSC-22-binding proteins in cells before and after DNA damage. ( A ): TSC-22-binding protein (arrow) in the nuclear fraction after DNA damage by phleomycin in cells expressing full-length TSC-22. a: Lamin-B1, Heat shock 70-kDa protein 1A/1B, b: Guanine nucleotide-binding protein-like 3 <t>(GNL3),</t> c: Pituitary adenylate cyclase-activating polypeptide type I receptor, d: Charged multivesicular body protein 4a, Histone H1.2, Heterogeneous nuclear ribonucleoprotein A1, rRNA 2′-O-methyltransferase fibrillarin, *Flag-TSC-22. ( B ): TSC22(86)-binding proteins (←) after DNA damage by phleomycin in a whole cell fraction of cells expressing TSC22(86). a: Keratin, type II cytoskeletal 1, Ubiquitin carboxyl-terminal hydrolase 47, Protein tyrosine phosphatase receptor type C-associated protein, b: Keratin, type I cytoskeletal 9, Keratin, type II cytoskeletal 1, c: Histone H1.4, d: Histone H1t, Histone H1.2, e: Actin, cytoplasmic 1, f: Actin, cytoplasmic 1, Histone H1.2, Glyceraldehyde-3-phosphate dehydrogenase, g: Histone H1.2, Dermcidin, h: 40S ribosomal protein S6, Histone H1.2, Dermcidin, 40S ribosomal protein S2, *Flag-TSC22(86).
    Anti Nucleostemin Ns Rabbit Pabs, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+gnl3/GNL3+Antibody/pmc07096428__41467_2020_15289_MOESM1_ESM-39-61-65
    Average 93 stars, based on 1 article reviews
    anti nucleostemin ns rabbit pabs - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    rabbit polyclonal antibodies  (Bethyl)
    93
    Bethyl rabbit polyclonal antibodies
    Identification of TSC-22-binding proteins in cells before and after DNA damage. ( A ): TSC-22-binding protein (arrow) in the nuclear fraction after DNA damage by phleomycin in cells expressing full-length TSC-22. a: Lamin-B1, Heat shock 70-kDa protein 1A/1B, b: Guanine nucleotide-binding protein-like 3 <t>(GNL3),</t> c: Pituitary adenylate cyclase-activating polypeptide type I receptor, d: Charged multivesicular body protein 4a, Histone H1.2, Heterogeneous nuclear ribonucleoprotein A1, rRNA 2′-O-methyltransferase fibrillarin, *Flag-TSC-22. ( B ): TSC22(86)-binding proteins (←) after DNA damage by phleomycin in a whole cell fraction of cells expressing TSC22(86). a: Keratin, type II cytoskeletal 1, Ubiquitin carboxyl-terminal hydrolase 47, Protein tyrosine phosphatase receptor type C-associated protein, b: Keratin, type I cytoskeletal 9, Keratin, type II cytoskeletal 1, c: Histone H1.4, d: Histone H1t, Histone H1.2, e: Actin, cytoplasmic 1, f: Actin, cytoplasmic 1, Histone H1.2, Glyceraldehyde-3-phosphate dehydrogenase, g: Histone H1.2, Dermcidin, h: 40S ribosomal protein S6, Histone H1.2, Dermcidin, 40S ribosomal protein S2, *Flag-TSC22(86).
    Rabbit Polyclonal Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+gnl3/GNL3+Antibody/10__1128_slash_mcb__00093___14-70-1-8
    Average 93 stars, based on 1 article reviews
    rabbit polyclonal antibodies - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification of TSC-22-binding proteins in cells before and after DNA damage. ( A ): TSC-22-binding protein (arrow) in the nuclear fraction after DNA damage by phleomycin in cells expressing full-length TSC-22. a: Lamin-B1, Heat shock 70-kDa protein 1A/1B, b: Guanine nucleotide-binding protein-like 3 (GNL3), c: Pituitary adenylate cyclase-activating polypeptide type I receptor, d: Charged multivesicular body protein 4a, Histone H1.2, Heterogeneous nuclear ribonucleoprotein A1, rRNA 2′-O-methyltransferase fibrillarin, *Flag-TSC-22. ( B ): TSC22(86)-binding proteins (←) after DNA damage by phleomycin in a whole cell fraction of cells expressing TSC22(86). a: Keratin, type II cytoskeletal 1, Ubiquitin carboxyl-terminal hydrolase 47, Protein tyrosine phosphatase receptor type C-associated protein, b: Keratin, type I cytoskeletal 9, Keratin, type II cytoskeletal 1, c: Histone H1.4, d: Histone H1t, Histone H1.2, e: Actin, cytoplasmic 1, f: Actin, cytoplasmic 1, Histone H1.2, Glyceraldehyde-3-phosphate dehydrogenase, g: Histone H1.2, Dermcidin, h: 40S ribosomal protein S6, Histone H1.2, Dermcidin, 40S ribosomal protein S2, *Flag-TSC22(86).

    Journal: International Journal of Molecular Sciences

    Article Title: Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry

    doi: 10.3390/ijms222010913

    Figure Lengend Snippet: Identification of TSC-22-binding proteins in cells before and after DNA damage. ( A ): TSC-22-binding protein (arrow) in the nuclear fraction after DNA damage by phleomycin in cells expressing full-length TSC-22. a: Lamin-B1, Heat shock 70-kDa protein 1A/1B, b: Guanine nucleotide-binding protein-like 3 (GNL3), c: Pituitary adenylate cyclase-activating polypeptide type I receptor, d: Charged multivesicular body protein 4a, Histone H1.2, Heterogeneous nuclear ribonucleoprotein A1, rRNA 2′-O-methyltransferase fibrillarin, *Flag-TSC-22. ( B ): TSC22(86)-binding proteins (←) after DNA damage by phleomycin in a whole cell fraction of cells expressing TSC22(86). a: Keratin, type II cytoskeletal 1, Ubiquitin carboxyl-terminal hydrolase 47, Protein tyrosine phosphatase receptor type C-associated protein, b: Keratin, type I cytoskeletal 9, Keratin, type II cytoskeletal 1, c: Histone H1.4, d: Histone H1t, Histone H1.2, e: Actin, cytoplasmic 1, f: Actin, cytoplasmic 1, Histone H1.2, Glyceraldehyde-3-phosphate dehydrogenase, g: Histone H1.2, Dermcidin, h: 40S ribosomal protein S6, Histone H1.2, Dermcidin, 40S ribosomal protein S2, *Flag-TSC22(86).

    Article Snippet: After electrophoresis, the blot was prepared by applying an electric current for 7 min using a Bio-Rad Trans-Blot Turbo transfer system and blocked with 2% bovine serum albumin (BSA, Fujifilm, Wako Pure Chemical)-TTBS for 1 h. The blotted membrane was incubated with rabbit anti-human TSC22D1 polyclonal antibody, mouse anti-human histone H1 monoclonal antibody (Genetex, Irvine, CA, USA), and rabbit anti-human GNL3 polyclonal antibody (Genetex) 1000-fold diluted with 2% BSA-TTBS at room temperature for 2 h. Then, washing with TTBS for 10 min was repeated three times, and the blots were incubated with 4000-fold diluted HRP-conjugated anti-rabbit or anti-mouse secondary antibody (Santa Cruz Biotechnology) at room temperature for 1 h, followed by washing with TTBS for 10 min three times and detection using ELC Plus (Thermo Fisher Scientific) and X-ray film (Fuji super RX, Fujifilm, Tokyo, Japan).

    Techniques: Binding Assay, Expressing

    Intracellular binding of TSC-22 (TSC22D1-2) with histone H1. IP-Western blotting using nuclear extract after DNA damage in Flag-TSC-22 (TSC22D1-2)-transfected cells. In detection using anti-histone H1 or anti-GNL3 antibody, histone H1 was clearly detected.

    Journal: International Journal of Molecular Sciences

    Article Title: Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry

    doi: 10.3390/ijms222010913

    Figure Lengend Snippet: Intracellular binding of TSC-22 (TSC22D1-2) with histone H1. IP-Western blotting using nuclear extract after DNA damage in Flag-TSC-22 (TSC22D1-2)-transfected cells. In detection using anti-histone H1 or anti-GNL3 antibody, histone H1 was clearly detected.

    Article Snippet: After electrophoresis, the blot was prepared by applying an electric current for 7 min using a Bio-Rad Trans-Blot Turbo transfer system and blocked with 2% bovine serum albumin (BSA, Fujifilm, Wako Pure Chemical)-TTBS for 1 h. The blotted membrane was incubated with rabbit anti-human TSC22D1 polyclonal antibody, mouse anti-human histone H1 monoclonal antibody (Genetex, Irvine, CA, USA), and rabbit anti-human GNL3 polyclonal antibody (Genetex) 1000-fold diluted with 2% BSA-TTBS at room temperature for 2 h. Then, washing with TTBS for 10 min was repeated three times, and the blots were incubated with 4000-fold diluted HRP-conjugated anti-rabbit or anti-mouse secondary antibody (Santa Cruz Biotechnology) at room temperature for 1 h, followed by washing with TTBS for 10 min three times and detection using ELC Plus (Thermo Fisher Scientific) and X-ray film (Fuji super RX, Fujifilm, Tokyo, Japan).

    Techniques: Binding Assay, Western Blot, Transfection

    Putative function of TSC-22 (TSC22D1-2) and TSC-22(86) (TSC22D1-3). ( A ): TSC-22 may promote the degradation of histone H1 through this interaction, and replacement of chromatin binding by HMGA2 may cause changes in the expression of genes that regulate cell proliferation and differentiation, apoptosis induction, and cell senescence. ( B ): GNL3 is known as nucleostemin and is found in the nucleolus. GNL3 interacts with p53 and MDM2. TSC-22 (TSC22D1-2) might influence the inhibitory action of GNL3 on tumor cell growth.

    Journal: International Journal of Molecular Sciences

    Article Title: Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry

    doi: 10.3390/ijms222010913

    Figure Lengend Snippet: Putative function of TSC-22 (TSC22D1-2) and TSC-22(86) (TSC22D1-3). ( A ): TSC-22 may promote the degradation of histone H1 through this interaction, and replacement of chromatin binding by HMGA2 may cause changes in the expression of genes that regulate cell proliferation and differentiation, apoptosis induction, and cell senescence. ( B ): GNL3 is known as nucleostemin and is found in the nucleolus. GNL3 interacts with p53 and MDM2. TSC-22 (TSC22D1-2) might influence the inhibitory action of GNL3 on tumor cell growth.

    Article Snippet: After electrophoresis, the blot was prepared by applying an electric current for 7 min using a Bio-Rad Trans-Blot Turbo transfer system and blocked with 2% bovine serum albumin (BSA, Fujifilm, Wako Pure Chemical)-TTBS for 1 h. The blotted membrane was incubated with rabbit anti-human TSC22D1 polyclonal antibody, mouse anti-human histone H1 monoclonal antibody (Genetex, Irvine, CA, USA), and rabbit anti-human GNL3 polyclonal antibody (Genetex) 1000-fold diluted with 2% BSA-TTBS at room temperature for 2 h. Then, washing with TTBS for 10 min was repeated three times, and the blots were incubated with 4000-fold diluted HRP-conjugated anti-rabbit or anti-mouse secondary antibody (Santa Cruz Biotechnology) at room temperature for 1 h, followed by washing with TTBS for 10 min three times and detection using ELC Plus (Thermo Fisher Scientific) and X-ray film (Fuji super RX, Fujifilm, Tokyo, Japan).

    Techniques: Binding Assay, Expressing